The DNMT1 Assay Kit is designed to measure DNMT1 activity for screening and profiling applications. The key to the DNM1 Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for DNMT1 detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT1 for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

TRDMT1, DNA

Kit comes in a convenient format, with a 96-well plate precoated with DNMT substrate, the antibody against 5-methylcytosine, the secondary HRP-labeled antibody, S-adenosylmethionine, DNMT1 assay buffer, and purified DNMT1 for 100 enzyme reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

COMPONENTS:

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Useful for studying enzyme kinetics and HTS applications.

Svedruzic, Z.M., Curr Med Chem. 2008;15(1):92-106.

Application Reference(s):
ATM mediates pRB function to control DNMT1 protein stability and DNA methylation (2013)

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit