Human Histone 3 (HIST1H3E), GenBank Accession No. NM_003532, a.a. 2-136(end) with N-terminal His-tag MW = 15.4 kDa, expressed in an E. coli expression system.

P68431

Histone 3.1, HIST1H3E

Histone H3.1 was coated onto a 96-well plate, and methylation by G9a was carried out at room temperature for 60 min. Methylated Histone H3.1 was detected using Chemiluminescence assay.

8 mM PBS pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 3 mM DTT.

Aqueous buffer solution

≥ 6 months at -80°C

Useful as a substrate for histone methyltransferase and acetyltransferase assays. Ideal for screening small molecular inhibitors of histone modifying enzymes for drug discovery and HTS applications.

1.   Loyola, A. et  al., Mol. Cell  24  (2), 309-316 (2006). 
2.  Hans,  F. and  Dimitrov,  S., Oncogene 20(24), 3021-3027 (2001).

Product Specific Reference(s):
1. Morita, S., et al., Peptides. 48, 75-82 (2013). 

Avoid freeze/thaw cycles

Methyltransferase/Substrate

Recombinant Protein