Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal His-tag, MW=60.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

AKT1, PKBα, PKBalpha, RACalpha, AKT

Inactive

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.2mM KCl, 10% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at –80°C

Useful as a substrate in kinase assays.

1. Alessi et al., Current Biology 8: 69, 1998.
2. Cohen et al., FEBS Lett. 410: 3, 1997.

Application Reference:
Arachidonoyl-phosphatidylcholine oscillates during the cell cycle and counteracts proliferation by suppressing Akt membrane binding (2013)

Avoid freeze/thaw cycles

Kinase

Recombinant Protein