Human Aurora kinase A also known as, AURKA, Serine/Threonine- Protein Kinase Aurora-A, and Breast Tumor-Amplified Kinase, GenBank Accession No. NM_003600, a.a. 2-403 (end) with N-terminal His-tag, expressed in a HEK 293 cell expression system. MW = 46 kDa.

O14965

AURKA, Serine/Threonine- Protein Kinase Aurora-A, Breast Tumor-Amplified Kinase

Aqueous buffer solution.

45 pmole/min/μg.

Assay was done in Kinase buffer containing 2 mM DTT using KEMPtide substrate (0.2 mg/ml) and 10 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using ADP-Glo reagent.

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 200mM Imidazole, 20% glycerol, and 3 mM DTT.

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Ouchi, M. et al., J. Biol Chem. 279 (19),19643-19648 (2004).
2. Kunitoku, N. et al., Dev. Cell 5(6), 853-864 (2003).

Avoid multiple freeze/thaw cycles

Kinase

Protein