产品名称 AMPK (A1/B2/G1), His-tag - Serine-Threonine Kinases - Kinase(s)
产品货号 40703
产品价格 现货询价,电话:010-67529703
产品规格 5 µg
产品品牌 bpsbioscience
产品概述
产品详情
Species: Human
Host Species: Sf9 insect cells
MW: ~144 kDa (complex)
Genbank #: NM_006251 (A1), NM_005399 (B2), NM_002733 (G1)
Tag(s): C-terminal His-tag
a.a: full length
Background:
AMPK (A1/B2/G1) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress (1). AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The β subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle (2).
Description:
Recombinant human AMPK (combination of A1/B2/G1 subunits), full length, with C-terminal His-tags, expressed in Sf9 insect cells via a baculovirus expression system. MW = 68 kDa (A1), 36 kDa (B2), 40 kDa (G1).
Synonym(s):
Subunit A1: PRKAA1, MGC33776, MGC57364
Subunit B2: PRKAB2, MGC61468
Subunit G1: PRKAG1, AMPKG, MGC8666
Purity:
≥75%
Formulation:
50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216–219.
2. Thornton, C. et al: Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle. J. Biol. Chem. 273: 12443-12450, 1998.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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