AXL (GenBank Accession No. NM_021913), a.a. 473-894 with N-terminal GST-tag, MW= 77 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

AXL, UFO, JTK11

One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 2 mM DTT using 0.2 mg/ml AXL Substrate and 20 mM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using ADP-Glo reagent.

5 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Sawabu T. et al., Mol Carcinog. 2007 Feb,46(2):155-64.
2. Vajkoczy P. et al., Proc Natl Acad Sci U S A. 2006 Apr 11,103(15):5799-804.

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein