Human Sphingosine kinase 2 (GenBank Accession No. AF245447), full length with N-terminal His-tag, MW=69.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

SphK2, sphingosine kinase 2, SPK2

20 U/µg

A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.

Sphingosine kinase activity was determined using D-erythro-sphingosine and ATP as substrates. Assay condition: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 4 µM D-erythrosphingosine, and 0.75 μg/ml sphingosine kinase 2 at 37°C for 30 min. ATP reduction is detected using Kinase-Glo^® Luminescent Kinase Assay Platform (Promega Corporation, Madison, WI).

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 20% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Zemann, B., et al., Blood.107(4):1454-8 (2006)
2. Maceyka, M., et al., J Biol Chem. 280(44):37118-29 (2005)

Application Reference:
1. Synthesis and biological evaluation of carbocyclic analogues of pachastrissamine (2015)
2. Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation (2014)

Avoid freeze/thaw cycles

Kinase/Lipids

Recombinant Protein