| 产品名称 | PI3 kinase (p110α/p85α), GST-tag - Lipid Kinase - Kinase(s) |
| 产品货号 | 40621 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 235 kDa (complex)
Genbank #: U79143 (p110α), XM_043865 (p85α)
Tag(s): N-terminal GST-tag(p110α), no tag on p85α
a.a: full length
Description: Complex of N-terminal GST-tagged recombinant full-length human p110α (GenBank Accession No. U79143), and recombinant full length, human p85α (no tag) (GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system. p110α MW=151 kDa, p85α MW=84 kDa.
Synonym(s): PIK3CA, p110alpha, Phosphoinositide-3-Kinase, Catalytic, Alpha, PIK3R1, Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha), GRB1, p85alpha
Specific Activity: 880 pmol/min/μg
Unit Definition: A unit of p110α/p85α kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.
Assay Conditions: A 25 μl kinase reaction is conducted in a buffer containing 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 5 mM MgCl2
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol, and 3 mM DTT.
Format: Aqueous buffer solution
Storage / Stability: >6 months at –80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Vogt, PK et al., Virology. 344(1):131-(2006)
2. Bader, AG et al., Nat Rev Cancer. Dec 5(12):921-9 (2005) Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Lipids
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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