Mouse Sphingosine kinase 1 (GenBank Accession No. AF415213), full length with N-terminal His-tag, MW=47.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

SphK1, sphingosine kinase 1, SPK1

20 U/µg

A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.

Sphingosine kinase activity was determined using D-erythro-sphingosine and ATP as substrates. Assay condition: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 4 µM D-erythrosphingosine, and 0.1-0.5 µg/ml sphingosine kinase 1 at 37°C for 30 min. ATP reduction is detected using Kinase-Glo^® Luminescent Kinase Assay Platform (Promega Corporation, Madison, WI).

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Delon, C., et al., J. Biol. Chem 279 (43) 44763-44774 (2004)
2. Taha, TA et al., FASEB J. 20(3):482-4 (2005)

Avoid freeze/thaw cycles

Kinase/Lipids

Recombinant Protein