Cyclin-dependent kinase 4 (CDK4) assembles with the ‘D’ type of Cyclins, e.g. cyclins D1, D2, and D3, and play a pivotal role in the cell cycle entering the S phase. Dysregulation of the activity of CDK4 is related to many different cancers, indicating CDK4 inhibitors as promising anticancer treatments. In fact, multiple CDK4 inhibitors, including Palbociclib, were approved by the FDA recently. The CDK4 Assay Kit is designed to measure CDK4/CyclinD3 activity for screening and profiling applications, using Kinase-Glo® Max as a detection reagent.
| 产品名称 | CDK4 Assay Kit - Kinase Assay Kits - Kinase(s) |
| 产品货号 | 79674 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 Reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Description: Materials Required But Not Provided: Kinase-Glo® Max Luminescence Kinase Assay (Promega #V6071) Dithiothreitol (DTT, 1 M; optional) Microplate reader capable of reading luminescence Adjustable micropipettor and sterile tips 30°C incubator Synonym(s): Cyclin-Dependent Kinase 4, Cell Division Kinase 4, CDK4
Supplied As: The CDK4 Assay Kit comes in a convenient 96-well format, with enough purified recombinant CDK4/CyclinD3 enzyme, CDK4 substrate peptide, ATP and kinase assay buffer for 100 enzyme reactions.
Format:
Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: Up to 6 months when stored as recommended.
Application(s): Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s): Uzma, A. et al., Nature Review Drug Discovery 14:130-146 (2015)
Warning(s): Avoid multiple freeze/thaw cycles.
Data shown is lot-specific. Contact us for specific information on other lots.
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