Bruton’s tyrosine kinase or BTK, is an enzyme that plays a role in the functionality and maturation of B cells. The BTK pathway has implications for a number of autoimmune disorders including isolated growth hormone deficiency type III and rheumatoid arthritis. The BTK Assay Kit is designed to measure BTK activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The BTK Assay Kit comes in a convenient 96-well format, with enough purified recombinant BTK enzyme, PolyGluTyr peptide, ATP, and kinase assay buffer for 100 enzyme reactions.

Kinase-Glo MAX (Promega #V6071)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

The BTK Assay Kit comes in a convenient 96-well format, with enough purified recombinant BTK enzyme, PolyGluTyr peptide, ATP, and kinase assay buffer for 100 enzyme reactions.

Up to 6 months when stored as recommended.

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Rawlings, D., Saffran, D., Tsukada, S., Largaespada, D., Grimaldi, J., Cohen, L., et al. (1993). Mutation of unique region of Bruton’s tyrosine kinase in immunodeficient XID mice. Science 261(5119): 358–361.

Avoid freeze/thaw cycles

Kinase

Assay Kit