B-raf, after stimulation by activated RAS, phosphorylates MEK1 resulting in activation of the mitogen-activated protein (MAP) kinase cascade. Since the MAPK cascade has been shown to be a critical factor in melanoma development, it has been proposed that targeting B-raf could be a promising approach to combat melanoma. More importantly, 40-60% of melanomas display a mutation in BRAF, e.g. B-Raf(V600E), which leads to constitutive activation of the MAP kinase pathway. The B-Raf(V600E) Kinase Assay Kit is designed to measure B-Raf(V600E) kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. In addition, the B-Raf(V600E) Kinase Assay Kit includes wild type B-Raf enzyme as a positive control for researchers investigating B-Raf(V600E) specific inhibitors.

BRAF1, RAFB1, B-raf 1, MGC126806, MGC138284, BRAF (V600E)

The B-Raf(V600E) Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant B-Raf enzyme, B-Raf substrate, ATP and kinase assay buffer for 100 enzyme reactions.

See assay kit data sheet for detailed protocol.

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Cantwell-Dorris, ER, et al. Mol Cancer Ther. 2011 Mar;10(3):385-94

Obaid NM, et al. Int J Mol Sci. 2017 Mar 8;18(3). pii: E585.

Avoid freeze/thaw cycles.

Kinase

Assay Kit