The Abelson (ABL) family of nonreceptor tyrosine kinases is critical to the progression and treatment of chronic myeloid leukemia (CML). The single-point mutation T315I has been linked to drug resistance and poor prognosis, making it an important drug target. The ABL (T315I) Assay Kit is designed to measure ABL activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The ABL (T315I) Assay Kit comes in a convenient 96-well format, with enough purified recombinant ABL enzyme, ABLtide peptide, ATP, and kinase assay buffer for 100 enzyme reactions.

Kinase-Glo MAX (Promega #V6071)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

Up to 6 months when stored as recommended.

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1. Greuber, E. K., Smith-Pearson, P., Wang, J., & Pendergast, A. M. (2013). Role of ABL Family Kinases in Cancer: from Leukemia to Solid Tumors. Nature Reviews. Cancer, 13(8), 559–571.

2. Ganguly SS, Plattner R. (2012). Activation of Abl Family Kinases in Solid Tumors. Genes & Cancer 3(5-6):414-425.

Avoid multiple freeze/ thaw cycles

Kinase

Assay Kit