| 产品名称 | ALK2, GST-tag - Kinase(s) |
| 产品货号 | 40019 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 67 kDa
Genbank #: NM_001105
Tag(s): N-terminal GST-tag
a.a: 147-510 (end)
Background: ALK 2 is a receptor serine/threonine kinase that is member of the ALK family and is upstream of signaling pathway involving the SMAD proteins especially SMAD1/5/8. Knockdown of ALK2, but not TGFβRI (ALK5), abrogates endoglin-mediated decrease in cell motility of prostate cancer cells and constitutively active ALK2 is sufficient to restore a low-motility phenotype in endoglin deficient cells (1). Therefore, endoglin decreases prostate cancer cell motility through activation of the ALK2-Smad1 pathway. ALK2 is the key gene involved in Fibrodysplasia ossificans progressiva (FOP), a rare autosomal dominant congenital disorder characterized by progressive heterotopic bone formation in muscle tissues (2).
Description: Recombinant human ALK2 a.a. 147-end (Genbank Accession No. NM_1001105) with an N-terminal GST-tag, expressed in Sf9 cells using a baculovirus expression system. MW = 67 kDa.
Synonym(s): ActRIA, ACVR1, ACTRI, ACVR1A, ACVRLK2, FOP, SKR1, TSRI, ALK
Specific Activity: 40 pmol/min/µg
Assay Conditions: Enzyme reactions include 40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA, 10 μM ATP, 0.2 mg/mL casein, 3 mM DTT, and various amounts of ALK2—incubated 45 minutes at 30 °C. Followed by addition of 25 μL of BPS ADP-Glo reagent—incubated 45 minutes at room temperature. Followed by addition of 50 µl of BPS Kinase Detection Reagent—incubated 45 minutes at room temperature. Luminescence was measured.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20 % Glycerol and 3 mM DTT
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Craft, C.S. et al . Oncogene. 2007 Nov 8;26(51):7240-50.
2. Shore, E. M. et al . Nature Genet. 38: 525-527, 2006. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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