The HSP90β (N-terminal) Assay Kit is designed for identification of HSP90β inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant HSP90β. The key to the HSP90β Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for HSP90β reactions. The fluorescently labeled geldanamycin is incubated with a sample containing HSP90β enzyme to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.

P08238

HSP90b, Heat Shock Protein 90b, HSP90beta, HSP90β

The HSP90β inhibitor screening assay kit comes in a convenient 384-well format, with purified HSP90β enzyme, fluorescently labeled geldanamycin and HSP90 assay buffer for 384 enzyme reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

See data sheet for detailed protocol.

Stable for at least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1. Kim, J., et al. J Biomol Screening 2004, 9(5): 375-381
2. Howes, R., et al. Anal Biochem 2006, 350: 202-213

Avoid freeze/thaw cycles

Heat Shock Proteins

Assay Kit