The HSP90α (C-Terminal Domain) TR-FRET assay kit is designed to measure the inhibition of HSP90α binding to its protein target PPID (also known as Cyclophilin D or Cyp40) in a homogeneous, 384 reaction format. This assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple. A sample containing terbium-labeled donor, dye-labeled acceptor, HSP90α, substrate, and an inhibitor is incubated for 2 hours. The protein-protein interaction is then assayed by measuring the TR-FRET between the PPID and HSP90α using a fluorescence reader.

P07900

HSP90a, Heat Shock Protein 90a, HSP90alpha, HSP90α, HTRF

Kit comes in a homogeneous, 384 reaction format

See assay kit data sheet for detailed protocol

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Allan, R.K. et al. J. Biol. Chem. 2006 281(11): 7161-71

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance
Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Avoid freeze/thaw cycles

Heat Shock Proteins/Assay Kits

Assay Kits