Human JMJD2D (GenBank Accession No. NM_018039), amino acids 2–354, with N-terminal GST tag, MW=67 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q6B0I6

KDM4D

≥ 0.14 pmole/min/μg

10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1 mM TCEP, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2D (5 – 25 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT and 20% glycerol

At least 6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Katoh, M., Int J Oncol. 2004 Jun; 24(6):1623-8.
2. Shin, S., et al., Biochem Biophys Res Commun. 2007 Feb 23; 353(4):973-7.

Application Reference(s):
A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Avoid freeze/thaw cycles

Demethylase

Recombinant Protein