Human JMJD1B, also known as lysine-specific demethylase 3B (KDM3B), JHDM2B, and KIA1082, GenBank Accession No. NM_016604/ NP_057688, a.a. 2-1761 (end) with Nterminal Avi- and FLAG-tags, MW=195 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

Q7LBC6

KDM3B, JHDM2B, KIA1082

0.0045 pmole/min/μg

Reaction was performed with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 μM -ketoglutarate, 25 μM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 μM biotinylated peptide substrate, and JMJD1B added to the wells. Add antibody against methylated H3K9. Incubate for 30 min. followed by Alphascreen® detection.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 μg/ml FLAG peptide

Aqueous buffer solution

At least 6 months at -80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Kim, J-Y, et al., Mol. Cel. Biol. 2012. 32(14):2917-2933.
2. Mikhaleva, I.I., et al., Peptides. 2011. 32(4): 826-831.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Demethylase

Recombinant Protein