The JARID1B Chemiluminescent Assay Kit is designed to measure JARID1B activity for screening and profiling applications. JARID1B is a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. The key to the JARID1B Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, JARID1B enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

JARID1B, PLU-1, KDM5B, Retinoblastoma-Binding Protein 2 Homolog 1, RBP2-H1, Jumonji, AT Rich Interactive Domain 1B

The JARID1B Chemiluminescent Assay Kit comes in a convenient format, with a 96-well strip plate pre-coated with methylated histone H3 peptide substrate, primary antibody, secondary HRP-labeled antibody, demethylase assay buffer, and purified JARID1B.

DMSO >1%, strong acids or bases, ionic detergents, high salt

See assay kit data sheet for detailed protocol

One year from date of receipt when stored as directed.

Great for studying enzyme kinetics and HTS applications.

Klose, R.J., et al. Cell 2007; 128: 889.

Avoid freeze/thaw cycles.

Demethylase

Assay Kit