The JARID1A Homogeneous Assay Kit is designed to measure activity of the JARID1A for screening and profiling applications, using AlphaLISA^® technology. The key to the JARID1A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1A enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

JARID1A, RBBP2, KDM5A, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen

The JARID1A Homogeneous Assay Kit comes in a convenient AlphaLISA^® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1A for 384 enzyme reactions.

Avoid green and blue dyes that absorb light in the Alphascreen^® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen^® assays.

COMPONENTS:

See assay kit data sheet for detailed protocol.

>6 months at –80°C

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1. DiTacchio L, Le HD, Vollmers C et al. Science 2011; 333(6051):1881-5.

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Avoid freeze/thaw cycles.

Demethylase

Assay Kit