The FBXL10 Chemiluminescent Assay Kit is designed to measure FBXL10 activity for screening and profiling applications. FBXL10, also known as KDM2B, JHDM1B, and CXXC2, is a histone lysine demethylase that exhibits demethylation activity toward trimethyllysine 4 (H3K4me3) and dimethyl-lysine 36 (H3K36me2) on histone H3. The key to the FBXL10 Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, FBXL10 enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

TBST buffer (1x TBS, pH 8.0, containing 0.05% Tween-20)

Luminometer or fluorescent microplate reader capable of reading chemiluminescence

Adjustable micropipettor and sterile tips

Rotating or rocker platform

KDM2B, JHDM1B, CXXC2

The FBXL10 Chemiluminescent Assay Kit comes in a convenient 96-well strip plate format, precoated with methylated histone H3 peptide substrate, primary antibody, secondary HRP-labeled antibody, demethylase assay buffer, and purified FBXL10 for 100 enzyme

DMSO >1%, strong acids or bases, ionic detergents, high salt

See product datasheet for detailed protocol.

One year from date of receipt when stored as directed.

Great for studying enzyme kinetics and HTS applications.

Iwase, S., et al. Cell 2007; 128(6):1077-1088.

Since January of 2019, this kit has been improved with reformulated blocking buffer and HRP substrates. The previous version of FBXL10 kit #50157 can still be purchased upon special request.

Avoid freeze/thaw cycles.

Demethylase

Assay Kit