ALKBH5 (AlkB Homolog 5, RNA Demethylase) is an enzyme that removes N6-Methyladenosine (m6A) from target mRNAs. ALKBH5 has been connected to various cancers, viral replication, and metabolic diseases while also being shown to remove the alkylation damage and restore adenine in DNA.
| 产品名称 | ALKBH5 Chemiluminescent Assay Kit - Assay Kits - Demethylase(s) |
| 产品货号 | 79659 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 reactions |
| 产品品牌 | bpsbioscience |
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| 产品详情 |
Background: Description: The ALKBH5 Chemiluminescent Assay Kit is designed to measure ALKBH5 activity for screening and profiling applications. The key to the ALKBH5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated substrate. Signal is inversely related to ALKBH5 demethylase activity. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, ALKBH5 enzyme is incubated with the substrate. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Materials Required But Not Provided: TBST buffer (1x TBS, pH 8.0, containing 0.05% Tween-20), prepared with DEPC-treated water Synonym(s): Human ALKBH5, AlkB Homolog 5, Alkylated DNA repair protein alkB homolog 5, RNA
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Format: COMPONENTS:
Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: 6 months from date of receipt, when stored as directed.
Application(s): Great for studying enzyme kinetics and HTS applications.
Reference(s): Zheng, G., Dahl, J.A., et al. 2013. ALKBH5 Is a Mammalian RNA Demethylase that Impacts RNA Metabolism and Mouse Fertility, Molecular Cell, 49(1):18-29. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Demethylase
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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