Human F-Box Protein 11 (FBXL11), also known as KDM2A, Ndy2, and JHDM1A GenBank Accession # NM_012308, a.a. 2-700 with N-terminal FLAGAvi- tag, MW = 82 kDa, expressed in Sf9 cells via a baculovirus expression system.

Q9Y2K7

F-Box Protein 11, KDM2A, Ndy2, JHDM1A

0.01 pmole/min/μg

The assay was performed using BPS Bioscience FBXL11 Homogeneous Assay Kit (Cat #50611) and following the manufacturer’s recommended protocol.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 μg/ml FLAG peptide

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Tanaka, Y., et al. (2010). EMBO J. 29(9):1510-22.
2. Blackledge, N.P., et al. (2010). Mol. Cell. 38(2):179-90.
3. Frescas, D., et al. (2008). Cell Cycle 7(22):3539-47.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Demethylase

Recombinant protein