Human F-Box Protein 10 (FBXL10, also known as KDM2B, JHDM1B, CXXC2, and Histone-H3 K36 demethylase 1B), GenBank Accession No. NM_032590, a.a. 1-650, with C-terminal FLAG-tag, MW= 75 kDa, expressed in Sf9 cells via a baculovirus expression system.

Q8NHM5

F-Box Protein 10, FBXL10, KDM2B, JHDM1B, CXXC2, Histone-H3 K36 demethylase 1B

≥83% by HPLC

Enzyme was incubated with 0.4 μM substrate in HEPES-containing JMJ assay buffer SA and SA- (without KG) in a 10-μl reaction for 180 min at 30˚C. 5 μl of Anti-Rabbit AlphaLISA® acceptor beads (1:250) and 5 μl of rabbit polyclonal antibody (1:200) were added and incubation continued for 30 min. Streptavidin Donor beads (1:125) were added (10 μl) and mixture was further incubated for 10 min at RT before Acounts were measured.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 µg/ml FLAG peptide

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Tanaka, Y., et al. (2010). EMBO J. 29(9):1510-22.
2. Blackledge, N.P., et al. (2010). Mol. Cell. 38(2):179-90. 3. Frescas, D., et al. (2008). Cell Cycle 7(22):3539-47.

Avoid freeze/thaw cycles.

Demethylase

Recombinant Protein