Human HDAC5 catalytic domain , GenBank Accession No. NM_005474, a.a. 656-1122 (end) with C-terminal His-tag, MW= 51 kDa, expressed in baculovirus expression system.

Q9UQL6

histone deacetylase 5, HDAC-5

≥1000 pmol/min/µg.

One U =1 pmol of acetyl group removed/min/µg of enzyme

25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 30 µM BPS HDAC class 2a substrate , BPS Catalog number 50040, and HDAC5. Incubation condition: 30 min at 30°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 imidazole, and 10% glycerol.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Spiegelberg, B.D. and Hamm, H.E. (2005) J. Biol. Chem. 280, 41769-41776
2. Waltregny et al., (2004) J. Neural Transm. 48, 273-290.

Application References:
1. An azumamide C analogue without the zinc-binding functionality (2017)
2. Impact of binding mechanism on selective inhibition of histone deacetylase isoforms (2017)
3. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014)
4. Identification and characterization of small-molecule inhibitors of lysine acetyltransferases (2014)
5. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)
6. The effect of various zinc binding groups on inhibition of histone deacetylases 1-11 (2014)
7. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013)
8. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013)
9. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013)

Avoid freeze/thaw cycles.

Deacetylase

Recombinant Protein