Human HDAC4 (GenBank Accession No. NM_006037), a.a. 627-1084 (end) with N-terminal GST-tag and C-terminal His-tag, MW= 76 kDa, expressed in baculovirus expression system.

P56524

histone deacetylase 4, HDAC-4

≥100,000 pmol/min/µg.

25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC4. Incubation condition: 30 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Leong, H. et al. (2005) Mol. Endocrinol. 19, 2930-2942.
2. Zhao et al., (2005) Mol. Cell. Biol. 25, 8456-8464.

Application Reference(s):
1. An azumamide C analogue without the zinc-binding functionality (2017)
2. Impact of binding mechanism on selective inhibition of histone deacetylase isoforms (2017)
3. Promiscuous actions of small molecule inhibitors of the protein kinase D-class IIa HDAC axis in striated muscle (2015)
4. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014)
5. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)
6. Thermodynamics of ligand binding to histone deacetylase like amidohydrolase from Bordetella/Alcaligenes (2014)
7. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013)
8. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013)
9. Highly ligand efficient and selective N-2-(Thioethyl)picolinamide histone deacetylase inhibitors inspired by the natural product psammaplin A (2013)
10. Imaging epigenetic regulation by histone deacetylases in the brain using PET/MRI with ¹⁸F-FAHA (2013)
11. Santacruzamate A, a potent and selective histone deacetylase inhibitor from the Panamanian marine cyanobacterium cf. Symploca sp (2013)
12. Selective class IIa histone deacetylase inhibition via a nonchelating zinc-binding group (2013)
13. Tasquinimod Is an Allosteric Modulator of HDAC4 survival signaling within the compromised cancer microenvironment (2013)

Avoid freeze/thaw cycles.

Deacetylase

Recombinant Protein