Human HDAC11 , full length, GenBank Accession No. NM_024827, with N-terminal GST-tag, MW= 66 kDa, expressed in baculovirus expression system.

Q96DB2

histone deacetylase 11, HDAC-11

≥3.7 pmol/min/µg.

25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC11 (15.6 - 2000 ng). Incubation condition: 35 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Strausberg, R.L. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002).

Application Reference(s):
1. Reassessing the effects of histone deacetylase inhibitors on hippocampal memory and cognitive aging (2014)

Avoid freeze/thaw cycles.

Deacetylase

Recombinant Protein