The Fluorogenic SIRT5 Assay Kit is a complete assay system designed to measure SIRT5 activity for screening and profiling applications. The Fluorogenic SIRT5 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only three simple steps on a microtiter plate are needed to analyze the SIRT5 activity level. First, the Sirtuin 5 fluorometric substrate (SIRT substrate 5), containing a succinylated lysine side chain, is incubated with purified SIRT5 enzyme. The desuccinylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

Q9NXA8

sirt5, sirtuin 5

The Fluorogenic SIRT5 Assay Kit comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT5 activity measurements.

DMSO >1%, strong acids or bases, ionic detergents, high salt

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1.J. Du et al. (2011) Science. 334: 806.
2. A. Madsen et al. (2012) J. Med. Chem 55: 5582.
3. F. Fisher et al. (2012) Plos One. 7: 1.

Avoid freeze/thaw cycles.

Deacetylase

Assay Kit