The Fluorogenic SIRT2 Assay Kit is a complete assay system designed to measure Sirtuin 2 (SIRT2) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 32 fluorescent SIRT2 activity measurements*.The kit includes purified SIRT2 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT2 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT2 activity level. First, the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated lysine side chain, is incubated with purified SIRT2 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

Q8IXJ6

sirt2, sirtuin 2

Kit comes in a convenient 96-well format, with all the reagents necessary for 32 fluorescent SIRT2 activity measurements.

DMSO >1%, strong acids or bases, ionic detergents, high salt

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1. A. Ito et al. (2001) EMBO J. 20 1331.
2. A. Ito et al. (2002) EMBO J. 21 6236.
3. N.A. Barlev et al. (2001) Mol. Cell 8 1243.

Application Reference(s):
Tubulin acetylation promoting potency and absorption efficacy of deacetylase inhibitors (2015)

Avoid freeze/thaw cycles.

Deacetylase

Assay Kit