The Kinetic HDAC assay developer is an essential reagent for signal generation in Kinetic HDAC assays performed with any of BPS Bioscience’s HDAC fluorimetric substrates. The developer sensitizes the deacetylated substrate so that subsequent treatment with the HDAC assay developer produces a fluorophore.

A 10x concentrate (1 ml) with 10 ml dilution buffer.

Aqueous buffer solution

Dilute 10x developer to 1x with dilution buffer before use. Add 50 µl 1x developer per 50 µl HDAC reaction (1:1, total volume 100 µl).

Store in aliquots, only as the 10x concentrate, at -70°C. Concentrate is stable for at least 3 freeze-thaw cycles, but care should be taken that both freezing and thawing steps are done quickly (e.g. liquid N₂ or dry ice/EtOH for freezing, rapid finger-rubbing for thawing).

Study of HDAC kinetics, regulation and inhibitor sensitivity. Suitable for use with the Kinetic HDAC assay kits:
• Kinetic HDAC1 Assay Kit
• Kinetic HDAC2 Assay Kit
• Kinetic HDAC3 Assay Kit

1. K. Ito et al. Proc. Natl. Acad. Sci. USA 2002 99: 8921
2. B. Heltweg and M. Jung Anal. Biochem. 2002 302: 175
3. S. Milutinovic et al. J. Biol. Chem. 2002 277: 20974

Avoid freeze/thaw cycles

Deacetylase

Kit Reagent