The PD-1:PD-L2[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to this kit is the high sensitivity of detection of biotin-labeled PD-L2 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L2 is incubated with PD-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

activity assay kit, Strep HRP, streptavidin

Kit comes in a convenient 96-well format, with biotin-labeled PD-L2, purified PD-1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt.

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

This kit is useful for screening for inhibitors of PD1 binding to PD-L2.

1. Molnar, E., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.

Avoid freeze/thaw cycles.

Cell Surface Receptor/Single-pass Transmembrane Protein

Assay Kit