Human Foxp3 luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter. 

Figure 1. Illustration of Foxp3 promoter region with representative transcription factor binding sites and enhancer regions.

Materials Required but Not Supplied for Cell Culture

• Thaw Medium 2 (BPS Bioscience #60184)
• Growth Medium 2B (BPS Bioscience #79530)

Materials Required but Not Supplied for Cellular Assay
• Assay Medium 2B (BPS Bioscience #79619)
• PMA (Fisher, Cat # BP685-1)
• Ionomycin (Fisher, Cat # BP25271)
• ONE-Step™ Luciferase Assay System (BPS Bioscience #60690)
• Luminometer
• 96-well tissue culture-treated white clear-bottom assay plate

Foxp3, Foxp3 cell line, scurfin cell line, foxo

Jurkat (Human Acute T-Cell Leukemic) Cell Line, Clone E61. Suspension cells.

This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Cat. #LT07-518) was used as a positive control.

Thaw Medium 2 (BPS Cat. #60184):  RPMI 1640 medium (Thermo Fisher, Cat. #A1049101) supplemented with 10% FBS (Thermo Fisher, Cat. #26140079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).

Growth Medium 2B (BPS Cat. #79530):  Thaw Medium 2 (BPS Cat. #60184) and 1 mg/ml G418 (Thermo Fisher, Cat. #11811031).

Each vial contains 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.

Store in liquid nitrogen immediately upon receipt.

The Foxp3 promoter Jurkat reporter cell line is suitable for monitoring the transcription activity of Foxp3 in response to stimulant, and establishing cell-based screens for inhibitors that target specific Foxp3-stimulating molecules.

Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Immunotherapy

Cell Line