The CRE/CREB Reporter (Luc) – Jurkat Cell Line contains a firefly luciferase gene under the control of multimerized cAMP response element (CRE) stably integrated into Jurkat cells. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. This cell line is validated for response to stimulation by Forskolin.

• Forskolin (Sigma, #F3917)
• Assay Medium: Thaw Medium 2 (BPS Bioscience, #60184)
• Growth Medium 2E (BPS Bioscience, #79638)
• 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
• One-Step luciferase assay system (BPS Bioscience, #60690)
• Luminometer

The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.

Thaw Medium 2 (BPS Bioscience #60184): RPMI1640 medium (Life Technologies, #A10491-01) supplemented with 10% FBS (Life Technologies, #26140-079), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).

Growth Medium 2E (BPS Bioscience #79638): Thaw Medium 2 (BPS Bioscience, #60184)  plus 0.5 μg/ml of Puromycin (Takara, #631306).

1. Montminy, M.R. et al. (1987) Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene. Nature 328(6126): 175-178. 

2. Fan Chung, K. (2006) Phosphodiesterase inhibitors in airways disease. Eur. J. Pharmacol. 533(1-3): 110-117. 

License Disclosure:

Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS Bioscience does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.