This liquid medium is optimized to culture select BPS Bioscience cell lines.

Cell media

RPMI 1640 medium (Thermo Fisher, #A1049101) supplemented with 10% FBS (Thermo Fisher, #26140079), 1mM Sodium pyruvate (Corning, #25-000-CL), 1% Non-essential amino acids (Corning, #25-025-CL), 1% Penicillin/streptomycin (Thermo Fisher, #15140122) T cell activation reagents (αCD3/αCD28 or Phytohaemagglutinin (PHA)).

Sterile filtered (0.2 μm) and Mycoplasma-free. Each lot is tested for growth of recombinant cells. All components are low endotoxin. FBS is ISIA-certified and sourced from the United States of America.

For detailed thawing and cell culture protocol, see cell line data sheet.

Upon receipt, the medium should be stored in the dark at 4°C. Avoid repeated freeze/thaw cycles. Do not exceed 37°C. When stored in the dark at 4°C, the product is stable for six months after first thaw.

Avoid repeated freeze/thaw cycles.

Cell Signaling Pathway

Media