The Firefly Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. This virus constitutively express firefly luciferase under a CMV promoter (Figure 1: Hygromycin and Figure 2: G418).

Two vials (500 μl x 2) of firefly luciferase lentivirus at a titer ≥5 x 106 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

The lentiviruses were produced from HEK293T cells. Supplied in medium containing 90% DMEM + 10% FBS.

See product datasheet.

Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freezethaw cycle.

Ideal as a positive control for transduction; useful for transduction optimization.

None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Cell Signaling Pathway

Lentivirus