| 产品名称 | NF-κB Reporter Kit (NF-κB Signaling Pathway) - Cell Signaling Pathway - Cell Based Assay Kits |
| 产品货号 | 60614 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 500 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: NF-κB (Nuclear Factor-KappaB, NFkB) is a heterodimeric protein composed of different combinations of members of the Rel family of transcription factors. The NF-kB /Rel family of transcription factors (p50, p65, c-Rel, etc.) are involved in stress, immune, and inflammatory responses. In unstimulated cells, the NF-κB dimers are sequestered in the cytoplasm by inhibitory IkB proteins. Proinflammatory cytokines, LPS, growth factors, and antigen receptors activate I B kinase (IKK), which phosphorylates the I B proteins. Phosphorylation of IkB leads to its degradation, freeing NF-κB complexes to translocate to the nucleus, bind to NF-κB DNA response elements, and induce the transcription of the target genes.
Description: The NF-κB Reporter kit is designed for monitoring the activity of the NF-κB signaling pathway in the cultured cells. The kit contains transfection-ready NF-κB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-κB responsive element located upstream of a minimal promoter. The NF-κB reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.
Synonym(s): NF-kB reporter, NFkappaB reporter, luciferase reporter, NF-kB reporter, NFkB reporter, Cell based assay kit, cell-based assay, reporter assay kit
Supplied As: Reporter: NF-κB luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector Concentration: Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each) Format: assay kit
Instructions for use: See data sheet insert for detailed transfection and assay protocols.
Storage / Stability: Stable at least 12 months from date of receipt, when stored as directed (-20°C) Application(s): • Monitor NF-κB signaling pathway activity.
• Screen for activators or inhibitors of NF-κB signaling or NF-κB pathway components. • Study effects of RNAi or gene overexpression on the activity of the NF-κB pathway. Reference(s): 1. Pessara, U., Koch, N. (1990). Mol Cell Biol. 10(8):4146-4154. Application Reference(s): Notes: The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.
Warning(s): Avoid freeze/thaw cycles
Product Type: Reporter Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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