The CRE/CREB Reporter kit is designed for monitoring the activity of the cAMP/PKA signaling pathway in cultured cells. The kit contains transfection-ready CRE luciferase reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response element (CRE) located upstream of a minimal promoter. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with the constitutivelyexpressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.

CRE reporter, CREB reporter, cAMP reporter, PKA reporter, cAMP response element, Cell based assay kit, cell-based assay, reporter assay kit

Reporter: CRE luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector

Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

assay kit

See data sheet for detailed transfection and assay protocols.

Stable at least 12 months from date of receipt, when stored as directed (-20°C)

• Monitor the JNK signaling pathway activity and AP1-mediated activity.
• Screen for activators or inhibitors of the JNK signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the JNK pathway.

1. Montminy, M.R. et al. (1987) Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene. Nature 328(6126):175-178
2. Fan Chung, K. (2006) Phosphodiesterase inhibitors in airways disease. Eur. J. Pharmacol. 533(1-3):110-117
3. Malik, R. et al. (2008) Cloning, stable expression of human phosphodiesterase 7A and development of an assay for screening of PDE7 selective inhibitors. Appl. Microbiol. Biotechnol. 77 (5): 1167-1173

Application References:
1. Nuclear TRAF3 is a negative regulator of CREB in B cells (2016)

The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.

Avoid freeze/thaw cycles

Cell Signaling Pathway

Reporter Assay Kit