ChIP-seq results obtained with the monoclonal antibody directed against ERalpha
ChIP was performed with the monoclonal antibody against ERalpha (Cat. # 25221) on sheared chromatin from MCF7 cells treated for 1 hour with estradiol. The immunoprecipitated DNA was analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1 shows the obtained peaks near the TFF1 gene on chromosome 21 (figure 1A), the GREB1 and HAAO genes on chromosome 2 (figure 1B and C), and the ZNF185 gene on the X-chromosome (figure 1D).

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