Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse G9a (Cat. No. 25224). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.

Western blot analysis using the antibody directed against G9a
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the antibody against mouse G9a (Cat. No. 25224), diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.