Determination of the antibody titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against Cre (cat. No. 25215) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,900.

Western blot analysis using the antibody directed against Cre (1)
Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the antibody against Cre (cat. No. 25215), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 38 kDa) is indicated on the right. 

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.