Western blot analysis using the antibody directed against ASH2
Figure 1A: Western blot was performed using the antibody against hASH2 (cat. #25209) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk on nuclear extracts from HeLa cells (upper panel: lane 1) and ectopically expressed FLAG-tagged ASH2 (ASH2-F, upper panel: lane 2). The lower panel shows the same western blot with an anti-FLAG antibody. The molecular weight marker is shown on the left.
Figure 1B: Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 μg) with the antibody against ASH2 (cat. #25209) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

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