Human GCN5, also known as KAT2A, GCNL2, or PCAF-β, GenBank Accession No. NM_021078, a.a. 362-837 (end), with C-terminal FLAG-tag, MW=55 kDa, expressed in a baculovirus infected Sf9 cell expression system.

Q92830

GCN5, KAT2A, GCNL2, PCAF-β, HAT, KAT

≥70 pmole/min/µg

Assayed with the BPS Bioscience Fluorogenic GCN5 Assay Kit, Cat. # 50091, per manufacturer's instructions.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide, and 20% glycerol

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Barlev, N.A., et al. (1998). Mol. Cell. Biol. 18 (3): 1349–58.
2. Wang, L., et al. (1997). Mol. Cell. Biol. 17 (1): 519–27.
3. Xu, W., et al. (1998). Mol. Cell. Biol. 18 (10): 5659–69.

Application Reference:
Quantitating the specificity and selectivity of Gcn5-mediated acetylation of histone H3 (2013)

Avoid freeze/thaw cycles.

Acetyltransferase

Recombinant Protein