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产品名称 P300 Chemiluminescent Assay Kit - Assay Kits - Acetyltransferase(s)
产品货号 79705
产品价格 现货询价，电话：010-67529703
产品规格 96 reactions
产品品牌 bpsbioscience
产品概述
产品详情
Description:

The p300 Chemiluminescent Assay Kit is an enzyme-linked immunosorbent assay (ELISA) designed to screen for inhibitors of p300 or to measure histone acetyltransferase (HAT) activity for screening and profiling applications. Histone acetyltransferase p300 (also known as KAT3B) is involved in various cellular events, and its dysfunction is linked to a number of human diseases including Parkinson’s, Huntington’s, and Alzheimer’s diseases. The P300 Chemiluminescent Assay Kit is based on the p300 enzyme transferring an acetyl group from an acetyl donor (acetyl CoA) to a histone substrate. The acetylated histone is recognized by a highly specific primary antibody, followed by an HRP-labeled secondary antibody. The chemiluminescence produced by HRP can be measured using a chemiluminescence reader.

P300 Chemiluminescent Assay Kit

Materials Required But Not Provided:

Tween-20
TBST buffer (1 x Tris-Buffered Saline, pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Synonym(s):
E1A Binding Protein P300, Histone Acetyltransferase P300, P300 HAT, Histone Crotonyltransferase P300, Protein Propionyltransferase P300
Supplied As:
The p300 Chemiluminescent Assay Kit comes in a convenient format, with Histone peptide, a 96-well plate precoated with Neutravidin and histone peptide, and all the reagents necessary for 96 chemiluminescent P300 activity measurements. In addition, the kit
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt.
Format:

Catalog

Component

Amount

Storage

50071

P300 human recombinant enzyme

5 μg

-80°C

Avoid freeze/thaw cycles!

 

Acetyl CoA (0.1 mM)

10 μl

-20°C

79708

2X HAT assay buffer

10 ml

-20°C

 

0.5 M DTT

20μl

-20°C

 79706

Primary antibody 327

12 μl

-20°C

52131H  

Secondary antibody 2

12 μl

-80°C

 

HRP chemiluminescent substrate A (transparent bottle)

6 ml

+4°C

 

HRP chemiluminescent substrate B (brown bottle)

6 ml

+4°C

52100

Blocking buffer

50 ml

+4°C

 

White microplate (or strips) precoated with Neutravidin and Histone Peptide

1 plate

+4°C
Instructions for use:
See assay protocol for detailed instructions.
Storage / Stability:
At least 6 months from date of receipt when stored as directed.
Application(s):
Great for studying enzyme activity and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

Ravindra, K. C., et al. (2012). Chem Res Toxicol. 25(2): 337–347.

Warning(s):
Avoid multiple feeze/thaw cycles.
Scientific Category:
Acetyltransferase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

79705.pdf
产品资料