Human Bromodomain containing 4, also known as BRD4, HUNK1, or MCAP, GenBank Accession No. NM_058243, a.a. 49-460 corresponding to bromodomains 1 and 2, with N-terminal His-tag, MW = 47.5 kDa, expressed in an E. coli expression system.

O60885

Bromodomain containing 4, BRD4, HUNK1, MCAP

BRD4 protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA^® ® Nickel Chelate acceptor beads (PerkinElmer) are added, followed by Alphascreen^®® Streptavidin -conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

45 mM Tris-HCl, pH 8, 124 mM NaCl, 2.4 mM KCl, and 10% glycerol.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

1. Rodriguez, RM, et al., J Mol Med (Berl). 2012 May;90(5):587-95.
2. Voigt, P, et al., Genome Biol. 2011 Nov 29;12(11):133.

Avoid freeze/thaw cycles.

Bromodomain

Recombinant Protein