Human Bromodomain containing 4, also known as BRD4, HUNK1, or MCAP, GenBank Accession No. NM_058243, a.a. 49-460 corresponding to bromodomains 1 and 2 with N-terminal GST-tag, MW = 73.3 kDa, expressed in an E. coli expression system.

O60885

Bromodomain containing 4, BRD4, HUNK1, MCAP

BRD4 protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA^® ® GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen^®® Streptavidin -conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 3 mM DTT.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Rodriguez, RM, et al., J Mol Med (Berl). 2012 May;90(5):587-95.

Avoid freeze/thaw cycles.

Bromodomain

Recombinant Protein