| 产品名称 | BCL-2, His-tag - PROTACs |
| 产品货号 | 50272 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 100 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 27 kDa
Genbank #: P10415
Tag(s): C-terminal His-tag
a.a: 1–211
Background: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Description: Human Bcl-2 (GenBank Accession No. P10415), a.a. 1-211 with C-terminal His-tag, MW = 27 kDa, expressed in an E. coli expression system.
UniProt P10415
Synonym(s): Bcl, Bcl2, B cell lymphoma 2
Assay Conditions: Following the
protocol for BPS Cat. #50222. Briefly,
BCL-2 was combined with BCL-2
peptide ligand in TR-FRET assay buffer
along with anti-His Tb donor and dyelabeled
acceptor. Varying concentrations
of ABT 199 were added and the reaction
was incubated at room temperature for 2
hours followed by fluorescent intensity
measurements.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Format: Aqueous buffer solution
Application(s): Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.
Reference(s): 1. Labi V. et al., Cell Death Differ. 2008 Jun;15(6):977-87.
2. Rong Y.P. et al., Mol Cell. 2008 Jul 25;31(2):255-65. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Apoptosis
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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