Rat PDE10A GenBank Accession No. NM_022236), a.a. 449-end with N-terminal GST-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q9QYJ6

PDE10, phosphodiesterase 10A

≥ 8339 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3' , 5' -cAMP to 5' -AMP per minute at 37°C.

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 1 mM MnCl₂, 200 µM cAMP, and 2.5 kU 5?- nucleotidase, 37°C, 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Siuciak, J.A. et al., Neuropharmacology 51 (2), 374-385 (2006).
2. Hu,H. et al., Eur. J. Neurosci. 20 (12), 3351-3363 (2004).

Avoid freeze/thaw cycles.

PDE

Recombinant Protein