| 产品名称 | Cereblon/DDB1/Cul4A/Rbx1 Complex - Proteins - Neuroscience |
| 产品货号 | 100329-1 100329-2 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg 50 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: HEK293
MW: Cereblon: 51 kDa; DDB1: 128 kDa; Cul4a: 87 kDa; Rbx1: 13 kDa
Genbank #: Cereblon: NM_016302; DDB1: NM_001923; Cul4a: NM_003589; Rbx1: NM_014248
Tag(s): Cereblon: N-terminal FLAG-tag; DDB1: N-terminal FLAG-tag; Cul4a: N-terminal His-tag; Rbx1: N-terminal His-tag
a.a: Cereblon: 2-442(end); DDB1: 1-1140(end); Cul4a: 2-759(end); Rbx1: 2-108(end)
Description: Cereblon/DDB1/Cul4A/Rbx1 complex of human Cereblon (GenBank Accession No. NM_016302) a.a. 2-442(end) with N-terminal FLAG-tag and MW= 51 kDa, human DDB1 (GenBank Accession No. NM_001923), a.a. 1-1140(end) with N-terminal FLAG-tag and MW= 128 kDa, human Cul4A (GenBank Accession No. NM_003589), a.a. 2-759(end) with N-terminal His-tag and MW= 87 kDa, human Rbx1 (GenBank Accession No. NM_014248), a.a. 2-108(end) with N-terminal His-tag and MW= 13 kDa, co-expressed in a HEK293 expression system.
UniProt Cereblon:Q96SW2; DDB1: Q16531; Cul4a: Q13619; Rbx1: P62877
Synonym(s): CRBN, Protein X 0001, MRT2A MRT2
Supplied As: Aqueous buffer solution.
Assay Conditions: PROTAC ternary complex formation with BRD3 and cereblon was assessed using AlphaScreen™ technology. Various amounts of Cereblon were incubated in 10-µl reaction with 100 nM dBET1 and 15 nM GST-BRD3 BPS #31033 at room temperature for one hour. After this, FLAG-acceptor beads and GSHdonor beads were added followed by detection of A-counts. Formulation: 40 mm Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20 and 20% Glycerol.
Storage / Stability: At least 6 months at –80°C.
Application(s): Useful for the study of protein degradation and PROTAC design, and selectivity profiling.
Reference(s): 1. Gang, Lu., et al., Science. 2014; 343(6168): 305-309. 2. Zhu, Y.X., et al., Blood. 2011; 118: 4771-4779. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Ubiquitin
Product Type: Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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