Quickgrow™ E. coli is specifically selected for its fast growth properties. Unlike some other commercial E. coli strains, which take at least 14 hrs for the colonies to grow large enough for picking, Quickgrow™ E. coli can grow to pickable colonies on LB plates within 9 hours. Quickgrow™ E. coli provide alpha-complementation of the beta-galactosidase gene for color selection. They may be used for producing single-stranded DNA from M13 or phagemid and are also useful for inducible protein expression of those genes under LacUV5 promoter control. The genotype is F' proA+B+ lacIq D(lacZ)M15/ D(lac-proAB) glnV thi-1 D(hsdS-mcrB)5.

Components:
20 vials (50 µl each) Quickgrow™ E. coli cells, ~1 x10⁹ cells/vial)
10 µl pUC18 control DNA (0.1 ng/µl)
10 ml SOC medium

1.0 x 10⁹ CPU (colonies per µg pUC18 DNA)

Transformations are performed with 50 aliquots of cells and 2 ul of pUC18 control plasmid (10 pg/ul) following the provided protocol. Dilute the reaction 1:100 with Super Optimal Broth with 20 mM glucose (SOC) at room temperature. 100 ul of the dilution is plated in duplicate on LB agar plates with 50 mg/ml ampicillin. The plates are incubated at 37°C overnight and the efficiency is calculated based on the average number of colonies per plate.

Aqueous buffer solution

See data sheet for detailed protocol.

Store competent cells at -80°C upon receipt. Avoid freeze/thaw cycles. Upon first thaw, store in single use aliquots. Stable for at least 6 months from date of receipt, when stored as directed.

Avoid freeze/thaw cycles

Mol. Biol. Competent Cells / Buffer

Cells