Pseudomonas putida formaldehyde dehydrogenase (FDH) a.a. 2 – 399 (end) (GenBank Accession No. D21201) with N-terminal His-tag, MW = 42.9 kDa, expressed in an E. coli expression system.

P46154

formaldehyde dehydrogenase, FDH

≥35 µmol/min/µg

The reaction was monitored by measuring NADH production by following absorbance at 340nm at room temperature for 5min. Buffer: pH 7.3, 20 mM Tris, 50 mM NaCl, 20 µM Fe+2, 500 mM a-ketoglutarate, 2 mM ascorbic acid, 0.01% Tween-20, and 1 mM NAD+.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Ito, K., et al., J Bacteriol. 1994;176:2483-2491.
2. Tanaka, K., et al., J Mol Biol. 2002;324:519-533.

Avoid freeze/thaw cycles.

Metabolic Enzymes-Dehydrogenase

Recombinant Protein